Nagham Shakir Turkie; Sarah Faris Hameed
Abstract
The research work exemplifies a sensitive, rapid, and simple procedure for measuring fluconazole using turbidity-continuous flow injection analysis for the generation of white precipitate ...
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The research work exemplifies a sensitive, rapid, and simple procedure for measuring fluconazole using turbidity-continuous flow injection analysis for the generation of white precipitate via the reaction of fluconazole with phosphotungestic acid, and detecting the attenuation of incident light caused by light colliding with the precipitate surface grain using the NAG-4SX3-3D analyzer to determine turbidity (0-180 ͦ). The linear range extended from 0.01-18 mmol.L-1 for fluconazole measurements were considerably lower than 0.5 RSD % for the repetition (n=6) for the concentration chosen (2, 13 mmol.L-1), with limit of detection=7.5342 ng/sample from the gradual dilution over the lowest concentration on the calibration graph , linear dynamic range (r = 0.9989), (correlation coefficient), percentage linearity (R2 percent = 99.79) traditional approaches (UV-spectrophotometric at λmax=260 nm with linear range (0.001-1) mmol.L-1, r=0.9987, R2=0.9973, R2%=99.73, and turbidimetric method with linear range (0.01-17) mmol.L-1 , r= 0.9869, R2=0.9740, R2%= 97.40 were compared with the suggested strategy. In comparison to the typical reference method's 10 mm irradiation, it was discovered, in addition to the technique's developed sensitivity and the use of minimal chemicals, that this approach is characterized by a dynamic system, which avoids precipitated particle setting during measurements. The results indicated that the developed method has a wide range of concentration with a high linearity and sensitivity. Furthermore, the continuous dilution in CFIA allows for the management of high or low concentrations, for a wider range of applications. The devised approach is believed to be the most acceptable for fluconazole molecule determination in pure and pharmaceutical formulations when compared with the reference methods. The method used in this research work is a pioneered developed approach and proved its success in determination of fluconazole in pure and pharmaceutical formulations.
