The aim of present study was to develop a simple, precise, accurate and reproducible spectrophotometric method for simultaneous determination of ramipril and amlodipine besylate by UV-visible spectrophotometer using the first order derivative method. According to our present knowledge, no first order derivative method was reported so far. Thus, in present study it was decided to carry out first order derivative method and it was validated in compliance with ICH (Q2 R1) guideline. Ramipril and amlodipine besylate showed absorbance at the working wavelength of 211.87 nm (zero crossing point of amlodipine besylate) and 254.34 nm (zero crossing point of ramipril) respectively using distilled water as a diluent. Linearity was established over the concentration range of 2-25 μg/mL and 2-50 μg/mL for ramipril and amlodipine besylate with correlation coefficient 0.999 and 0.998 respectively. Accuracy was obtained between 99.91-101.06% and 99.66-100.66% for ramipril and amlodipine besylate respectively. LOD were found to be 0.078 μg/mL and 0.059 μg/mL and LOQ were 0.239 μg/mL and 0.178 μg/mL for ramipril and amlodipine besylate respectively. The results revealed that the developed method is suitable for the routine analysis of determining of ramipril and amlodipine besylate in a tablet dosage form.