Document Type : Original Article

Authors

Department of Biochemistry, Payame Noor University, 81581-84431, Isfahan, Iran

10.33945/SAMI/CHEMM.2020.2.7

Abstract

Spirulina platensis is a green-blue microalgae and a rich source of many nutrients such as protein, vitamins, and minerals. Spirulina contains peptides that have therapeutic and beneficial effects on the human body. In this study, peptide Ile-Gln-Pro from spirulina platensis was extracted using alcalase enzyme and purified by ultrafiltration and silica gel columns, and the fractions were identified using RP-HPLC analysis. The molecular weight of the tripeptide was determined by GC/mass spectroscopy. The peptide extracted with a concentration of 6.23 mg/mL and a purity of 75.72%. Then its inhibitory effect on angiotensin I-converting enzyme (ACE) and its anti-oxidant properties were investigated using DDPH (2,2-diphenyl-1-picrylhydrazyl) assay.

Graphical Abstract

Investigation of ACE Inhibitory Effect and Antioxidant Activity of Peptide Extracted from Spirulina Platensis

Keywords

[1] Samarakoon K., Jeon Y.J. Food Res. Int., 2012, 48:948
[2] Yu J., Hu Y., Xue M., Dun Y., Li S., Peng N., Liang Y., Zhao S. J. Microbiol. Biotechnol., 2016, 26:1216
[3] Liu J., Jin Y., Lin S., Jones G.S., Chen F, Food Chem., 2015, 175:258
[4] Wang L.S., Huang J.C., Chen Y.L., Huang M., Zhou G.H. J. Agric. Food Chem., 2015, 63:3437
[5] Chen N., Yang H., Sun Y., Niu J., Liu S. Peptides, 2012, 38:344
[6] Zhuang H., Tang N., Yuan Y. J. Funct. Foods, 2013, 5:1810
[7] Ondetti M.A., Cushman D.W. Biochem, 1982, 51: 283
[8] Skeggs L.T., Kahn J.R., Shumway N.P. J. Exp. Med., 1952, 95:241
[9] Suetsuna K. J. Nutr. Biochem., 1998, 9:415
[10] Lu J., Ren D.F., xue Y.L., Sawano Y., Miyakawa T., Tanokura M. Food Chem., 2010, 58:7166
[11] Bhuyana B.J., Mugesh G. Org. Biomol. Chem., 2011, 9:5185
[12] Bagatini M.D., Martins C.C., Battisti V., Gasparetto D., da Rosa C.S., Spanevello R.M., Ahmed M., Schmatz R., Rosa M., Schetinger C., Morsch V.M. Heart Vessels, 2011, 26:55
[13] Ray P.D., Huang B.W., Tsuji Y. Cell. Signal, 2012, 24:981
[14] Toeroek J. Physiol. Res., 2008, 57:813
[15] Mattson M.P. Exp. Gerontol., 2009, 44:625