Introduction

Amino alcohols, like 2-[(2-aminoethyl)amino]ethanol [1,2], are important in substitution reactions because they can act as both nucleophiles and leaving groups. In alkoxy phosphazene chemistry, this type of ligand can help create a range of structural motifs through its involvement in substitution reactions. It can donate its amino group as a nucleophile and also serve as a leaving group during the reaction, allowing for the formation of different substituted products with diverse structural properties [3-6]. When subjected to a reaction with hexachlorocyclotriphosphazene, this ligand exhibits a pronounced inclination toward the formation of spiro products [7,8]. This transformation entails the nucleophilic deprotonation of the amino group, leading to the displacement of two chlorine atoms on the same phosphorus atom of the phosphazene trimer by hydroxyl groups [9]. The size and geometry of the appended rings in the resultant cyclotriphosphazene structure are contingent upon the length of the difunctional nucleophilic reagents employed.

The family of substituted cyclotriphosphazenes holds a prominent position in the realm of inorganic heterocyclic systems, offering a versatile array of applications, encompassing drug delivery systems [10], lubricant [11,12], flame retardant [13-15], chemo-sensors [16-18], and anticancer agents [19-21]. Hexachlorocyclotriphosphazene (N₃P₃Cl₆) is a cyclic compound, can be used to make various types of polymers with special properties [22]. Some of these polymers are useful for making fire-resistant [22], biocompatible, and optoelectronic materials [22]. Hexachlorocyclotriphosphazene also has other applications, such as sterilization, fertilization, and cancer treatment. In the context of this study, we successfully synthesized a fully substituted phosphazene compound with twisted octahedral rings through a solvothermal reaction. Specifically, 2-[(2-aminoethyl)amino]ethanol (AEAE) was reacted with hexachlorocyclotriphosphazene (HCCTP) in the presence of triethylamine at a temperature of 70 °C using anhydrous toluene as the solvent for a duration of 9 days. The ratio of HCCTP to AEAE utilized in the reaction was 1:6. The structural characterization of this compound was accomplished through a comprehensive analysis employing ¹H, ¹³C, and ³¹P-NMR spectroscopy, complemented by IR spectroscopy. Furthermore, the spectral features related to light absorption were examined via fluorescence and ultraviolet spectroscopy.

To gain insights into the spatial geometry, symmetry, bond lengths, and angles within the synthesized compound's liquid state, we conducted density-functional theory (DFT) optimization and frequency calculations at the M06-2x/6-31G level. Comparison was made between the experimental IR spectrum and the IR spectrum obtained through computational simulations. In addition, the compound's photoelectron properties were probed in the context of its potential role as a chemo-sensor for Pb²⁺, Cd²⁺, Cu²⁺, and Ni²⁺ ions in a solvent mixture of water and alcohol (methanol) at a ratio of 1:9 (v/v), a configuration that capitalizes on the notable fluorescence characteristics of the spiro-alkoxy compound in DMSO solvent.

The biocompatibility of the synthesized compound was evaluated via the MTT assay, a widely accepted colorimetric method for assessing cell metabolic activity and viability 1, confirming its safety. Finally, the antineoplastic drug TAEAEAP, employed in cancer treatment, underwent molecular docking studies, interacting with molecules 1BD8 (CDK6 monomeric complex with an inhibitor) [23]  and 3NUP (structure of inhibitor P19nk4d) [24].

This study focused on the synthesis, structural analysis, and antibacterial properties of a purple-colored complex, Bis[tris(2-ethylaminoethyl)amine]copper(II)dinitrate ((TAEAEAP)₂Cu(NO₃)₂). The complex was formed through the reaction between copper nitrate and TAEAEAP in reflux conditions using 97% ethanol as the solvent for 4 hours. The formation of this purple complex occurred over several months in a dark environment and at ambient temperatures. Structural characterization was performed using analytical techniques such as IR spectroscopy, CHN analysis, and atomic absorption spectroscopy. Furthermore, the antibacterial efficacy of the copper complex against Gram-posetive Staphylococcus aureus ATCC 6538 (G+), and Bacillus subtilis 2a (ATCC 23857 G (+)) was also examined.

Experimental

Chemical preparation

In the experimental section, we utilized analytical-grade reagents for all chemicals and solvents. The L929 fibroblast cells were sourced from Pasteur Bank in Tehran. The infrared (IR) spectrum was recorded on a Thermo Nicolet FT-IR spectrometer using KBr tablets within the range of 400-4000 cm^-1. UV-Visible (UV-Vis) data were measured using a Shimadzu UV-VIS 2550 spectrophotometer Labomed, INC UVD2950 at ambient temperature, covering a range of 190-1100 nm. For nuclear magnetic resonance (NMR) analysis, a Bruker Avance DPX 250 instrument operating at 2550a MHz was employed to obtain ¹H-NMR, ¹³C-NMR, and ³¹P-NMR spectra. Fluorescence measurements were conducted utilizing a PerkinElmer LS45 Fluorescence Spectrometer. In addition, the antibodies and antigens of the infectious agent were detected using an Epoch Eliza reader from Bio-Rad. Elemental analysis of CHN was performed using a Thermo Finning Flash Elemental Analyzer (1112 EA) (Thermo Fisher Scientific Inc., Waltham, MA, USA). The melting point was measured on an electrically heated Barnsted Electrothermal 9200 apparatus.  Staphylococcus aureus ATCC 6538 (G+), and Bacillus subtilis 2a ATCC 23857 (G+) were obtained from the Pasteur Bank, Tehran. In the preparation of the material for this study, the measurement of copper (Cu) content was conducted using the MGA-1000 lumex Atomic Absorption Spectrometer.

Synthesis of (8s,10s,19s)-1,11,20-trioxa-4,7,9,14,17,18,23,26,27-ninaaza-8χ⁵ ,10χ⁵ ,19χ⁵ -triphosphatrispiro[7.1.7¹⁰.1 .7¹⁹ .1⁸ ]heptacosa-8(27),9,18-triene [N₃P₃(OC₂H₅NC₂H₅N)₃]

 In accordance with Equation 1, a solution was prepared by dissolving 0.3 g (3 mmol) of 2-[(2-aminoethyl)amino]ethanol  in 30 mL of anhydrous toluene. Then, 1.3 mL (6 mmol) of triethylamine was added gradually while stirring the amino-alcohol solution on a magnetic stirrer. After approximately 15 minutes, 0.03 g (1 mmol) of N₃P₃Cl₆ was added to 25 mL of toluene in the same way. The mixture was heated to 70 °C under anhydrous conditions for 9 days in a round-bottom flask with a glass stopper. The clear liquid separated from the dark yellow oily precipitate. The reaction solution was carefully evaporated using a rotary evaporator to yield a viscous dark yellow oil. Subsequently, this product was dissolved in dichloromethane (DCM) and subjected to two successive extractions with 10 mL of distilled water each. Through this process, the transfer of triethylamine hydrochloride occurred from the organic phase to the aqueous phase, facilitating its removal from the final product. After evaporation of DCM, pale-yellow oil was obtained. The yield was ~ 0.16 g. (36%). ³¹P-NMR (DMSO, ppm): δ 0.52 (s, P). ¹H-NMR (DMSO, ppm): δ 2.25 (p, 1H, ⁵J_H-H = 4.4 Hz, NH); 2.66-2.41 (m, 4H, CH₂-NH-CH_2, NH-CH₂), 2.97 (s, 2H OH₂); 3.40 (t, 1H, ³J_H-H = 5.8 Hz, NH). ¹³CNMR (DMSO, ppm): δ 43.46-34.33 (m, CH₂-O), 52.43 (d, J = 44.2 Hz, CH₂-NH); 60.85 (s, CH₂-NH). FTIR (KBr, cm^-1): ν 3442 (NH), 2959, 2860 (C-H), 1598 (NH bend), 1463 (CH bend), 1287, 1123, 958 (P=N), 1072 (C-N), and 742 (NH wag). UV-Vis (DMSO, nm): λ_max 295, 345. PL (DMSO, nm): λ_max 488.

(NPCl₂)₃ + 3(OH-(CH₂)₂-NH-(CH₂)₂NH₂) + 6 Et₃N → [NP(O-(CH₂)₂NH-(CH₂)₂NH)₃] + 6 Et₃N.HCl            (1)

Synthesis Bis [tris(2-ethylaminoethyl)amine]copper(II)dinitrate [[N₃P₃(OC₂H₅NC₂H₅N)₃]₂Cu(NO₃)₂]

Based on Equation 2, a dark blue solution was prepared by combining 2 mmol (equivalent to 0.8 g) of the ligand TAEAEAP with 1 mmol (equivalent to 0.24 g) of copper(II) nitrate in 30 cc of 98% ethanol. The mixture was allowed to react at room temperature conditions for 30 minutes, followed by a reflux period of 5 hours at 72 ºC. Subsequently, the solution was subjected to filtration and solvent extraction, resulting in the formation of a viscous, blue precipitate at the bottom of the vessel. Over a period of six months, a small purple mass gradually emerged at the precipitate's base. This material was subsequently purified through washing with methanol and subjected to drying. The paper solid yield was (0.8g). (64%), m.p = 195º. FTIR (KBr, cm^-1): ν 3309 (NH), 3254, 3217 (C-H), 1592 (NH bend), 1444 (CH bend), 1407(NO₃), 1107, 1060, 686 (P=N), 1085(C-N), and 980 (NH wag). Anal. Calcd. for C₂₄H₆₀CuN₂₀O₁₂P₆: C, 26.93; H, 5.65; N, 26.17; Found: C, 27.43.10; H, 7; and N, 28.2.

[NP(O-(CH₂)₂NH-(CH₂)₂NH)₃]₂ +  MX₄ → [[NP(O-(CH₂)₂NH-(CH₂)₂NH)₃]_2.. MX₂] +X₂                                     (2)

Preparation of stock solution of [N₃P₃(OC₂H₅NC₂H₅N)₃] and metal ions

Distilled water was employed to create standard stock solutions of TAEAEAP at a concentration of 1 mM and metal chloride at a concentration of 0.001 M. Subsequently, 0.1 ml of the 1 mM TAEAEAP solution was introduced into a 10 ml volumetric flask, and then diluted with a mixture of distilled water and methanol in a ratio of 9:1. Finally, the TAEAEAP solution was supplemented with 10 equivalents of metal ion salts to initiate the fluorescence detection process.

Computational Methodology

In the experimental section, we employed Gaussian09 [25] and GaussView [26] software to elucidate the structure of the synthesized product in its solid state, optimize its geometry, determine its symmetry, and measure bond lengths and angles. Density functional theory (DFT) structural optimization and frequency calculations were carried out using the M06-2x functional and the 6-31G basis sets, implemented within the Gaussian package [27]. 

For the docking procedure, we utilized AutoDock Vina v.1.2.0 in conjunction with Chimera and Discovery Studio. AutoDock Vina, an open-source application for molecular docking, was developed and implemented by Dr. Oleg Trott at The Scripps Research Institute's Molecular Graphics Lab (now CCSB). UCSF Chimera, created by the University of California, San Francisco's Resource for Biocomputing, Visualization, and Informatics (www.rbvi.ucsf.edu/chimera), received funding from the National Institutes of Health (P41-GM103311). BIOVIA[28], BIOVIA 27, developed by Dassault Systems, is a comprehensive molecular modeling application for visualizing, sharing, and analyzing data related to proteins and small molecules. It is integrated into Discovery Studio, San Diego, enabling seamless collaboration among experts and colleagues without the loss of scientific knowledge or time. The structural information for transferase/transferase inhibitor 3NUP (CDK6 monomeric in complex with inhibitor, PDB DOI: 10.2210/pdb3NUP/pdb) and tumor suppressor 1B8D (structure of CDK inhibitor P19INK4D, PDB DOI: 10.2210/pdb1BD8/pdb) was obtained from the Protein Data Bank (https://www.rcsb.org).

Antibacterial activity

This study aimed to assess the antibacterial efficacy of compound, [[N₃P₃(OC₂H₅NC₂H₅N)₃]₂Cu(NO₃)₂] against bacterial strains, specifically Staphylococcus aureus ATCC 6538 (G+), and Staphylococcus aureus ATCC 6538 (G+). The bacterial strains were sourced from the American Type Culture Collection (ATCC) and cultivated in Luria-Bertani (LB) broth under standardized conditions. The compounds were prepared in various concentrations and tested using the zone-of-inhibition method. Results were quantified by measuring the diameter of the inhibition zones around each compound-impregnated disk, with data analysis performed using Image J software [29]. This research provides valuable insights into the antibacterial potential of compounds III and IV against these two bacterial species.

Results and Discussion

In our synthesis, we successfully synthesized tris-bis(triazaphosphorine ethylenediamine) (TAEAEAP) by cyclizing (HCCTP) in a 1:3 ratio with the bifunctional N/O donor, 2-[(2-aminoethyl)amino]ethanol. This reaction was conducted in an anhydrous toluene environment with triethylamine serving as the hydrogen chloride acceptor. Notably, the cyclization proceeded with spiro-regiospecificity, as illustrated in Scheme 1. The copper complex, tris-bis(triazaphosphorine ethylenediamine)Cuprate (II)-di-nitrate(TAEAEAP)₂Cu(NO₃)₂, was subsequently synthesized in a 1:2 ratio using Cu(NO₃)₂·2H₂O and TAEAEAP in 98% ethanol. The structural representation of the copper complex is presented in Scheme 2.

Scheme 1: Mechanism of TAEAEAP synthesis through the HCCTP cyclization in a 1:3 ratio with 2-(2-aminoethyl) amino ethanol, using dry toluene and triethylamine as the hydrogen chloride acceptor. The cyclization proceeds with spiro-regiospecificity

Scheme 2: The Synthetic mechanism of bistris(2-ethylaminoethyl)aminecopper(II)dinitrate. This synthesis is achieved through the reaction of Copper (II) nitrate with TAEAEAP in a 2:1 ratio, specifically in ethanol with a purity of 98%

NMR results

In our analysis, we acquired ³¹P-NMR, ¹H-NMR, and ¹³C-NMR spectra of TAEAEAP dissolved in dimethyl sulfoxide (DMSO) at a temperature of 300 °C. The ³¹P NMR spectrum displayed a singlet signal at δ = -0.52, indicating the coupling between hydrogen and phosphorus in the A3 spin system, as illustrated in Figure 1. These spectra provided conclusive evidence that the compound had been fully substituted by amino alcohol, with all three phosphorus atoms residing in identical chemical environments. The amines within the phosphazene ring substituents manifested as pentets (δ = 2.25, J = 4.4 Hz, 1H) and triplets (δ = 23.40, J = 5.8 Hz, 1H).

In the ¹H-NMR spectrum, as depicted in Figure 2, it was observed that the compound featured two intermolecular carbon-hydrogen bonds with distances of 2.74 and 2.89 Å, respectively. Furthermore, intramolecular hydrogen bonds involving the oxygen atoms were evident, represented by a singlet peak at 2.79 ppm, as demonstrated in Figure 3.  The ¹³C-NMR spectrum in Figure 4 revealed two distinct signals: a doublet at 33.89 ppm and a multiple in the 46-47 ppm range. These signals strongly suggested the presence of hydrogen bonds formed between the oxygen atoms and the hydrogens attached to the carbons.

Infrared Spectroscopy

The IR spectra of the product and the precursors in Figure 5 show the presence of the phosphazene ring by the peak in the region 1287.91, 1123.08 cm^-1, which corresponds to the asymmetric stretching vibrations of P=N. The symmetric stretching of P-N gives another peak in the region of 958 cm^-1. The OH peak in the product is absent, and the peak in the region of 3442.86 is due to the amino group. The lack of the phosphorus-chlorine signal between 621.33 and 524.42 cm^-1 indicates that amino alcohols have completely replaced chlorine on phosphorus. [30].

In the analysis of the IR spectrum for the (TAEAEAP)₂Cu(NO₃)₂complex, two distinctive absorption bands were observed, both associated with the P=N group, occurring at 1107 cm^-1 and 1060 cm^-1. Notably, these bands appeared at lower wavenumbers when compared to TAEAEAP, indicating structural differences in the complex. Furthermore, a comparison of the υ (NH) vibrational frequency in the copper complex with the free TAEAEAP ligand revealed a notable shift of this vibration by 33cm^-1 towards higher frequencies upon complexation. In addition, an absorption band in the 1407 cm^-1 region was identified, which is attributed to the nitrate group. Detailed information on the IR absorption bands for the four compounds, namely HCCP, TAEAEAP, (AEAE), and (TAEAEAP)₂Cu(NO₃)₂, is provided in Table 1 for reference.

Table 1 presents the characteristic absorption bands, including P=N group vibrations, υ (NH) vibrational frequencies, and nitrate group-related frequencies, as observed in the IR spectra of the specified compounds.

Figure 1: ³¹PNMR spectrum of TAEAEAP

Figure 2: ¹H-NMR spectrum of TAEAEAP

Figure 3: Intra hydrogen bonds in the TAEAEAP structure were calculated with chimera and discovery studio

Figure 4: ¹³C-NMR spectrum of TAEAEAP

Figure 5: IR spectra of AEAE, TAEAEAP, HCCTP, and (TAEAEAP)₂Cu(NO₃)₂, respectively

Table 1: Presents the infrared spectroscopic absorption bands for HCCP, TAEAEAP, (AEAE), and (TAEAEAP)₂Cu(NO₃)₂. Furthermore, it includes a comparison of the absorption intensities to assess the cadence of absorption for the specified compounds

Ultraviolet and fluorescence studies

The UV spectrum of TAEAEAP in DMSO at a concentration of 4×10^-7 mol/ml and room temperature is depicted in Figure 6. It shows two absorbance bands at 295 nm and 345 nm. The band at 295 nm is related to π-π* transitions of the phosphazene ring. The replacement of chlorine by 2-[(2-aminoethyl) amino] ethanol on the phosphazene ring leads to a red shift of P=N, resulting in the band at 295 nm. The band at 345 nm may be attributed to intramolecular charge transfer within the 2-[(2-aminoethyl) amino] ethanol moiety attached to the phosphazene ring [31]. In the UV analysis of the complex, conducted in an aqueous solvent, three distinct absorption bands were observed at wavelengths of 194.8 nm, 255.6 nm, and 871.6 nm. The 871.6 nm band was attributed to d-d transitions within the complex. Therefore, by changing the solvent polarity from DMSO to water, the absorption bands of TAEAEAP shifted to less visible wavelengths, indicating a change in the electronic transitions occurring within the molecule. The fluorescence properties of the oily cyclotriphosphazene derivatives were examined in DMSO using diluted solutions at a concentration of 4×10^-7 mol/ml. A fluorescence band was observed at approximately 488 nm [30] (Figure 7).

Figure 6: UV Spectrum of TAEAEAP in DMSO at a concentration of 4×10^-7 mol/ml and UV-Visible Spectra of the complex (TAEAEAP)₂Cu(NO₃)₂ in aqueous solution at a concentration of 4×10-7 mol/m

Figure 7: Fluorescence spectrum of compound TAEAEAP (4x10^-7 M, excited at 400 nm) in DMSO

Atomic absorption

Based on the results obtained from atomic absorption analysis, it is evident that the sample under investigation, which was in powder form, underwent acid dissolution for analysis. The focus of this analysis was on the determination of copper (Cu) content within the sample. The results indicate that the sample contains approximately 20% copper, expressed in terms of elemental percentage. It is important to note that these results are reported without considering the potential effects of the sample matrix. The accuracy and reliability of these findings are contingent on the methodology employed and the sample preparation process.

Computational Studies

The structure obtained from density functional theory (DFT) calculations is demonstrated in Figure 8. The structure comprised heterocyclic molecules attached to the N₃P₃Cl₆ core through distorted 8-membered rings. Due to steric effects, the structure exhibited non-planarity and deviation. It exhibited C1 symmetry and possessed estimated stability energy of approximately 5821753.473 KJ. Bond lengths and angles between the atoms are presented in Table 2. Comparisons were made between the experimental infrared spectrum of TAEAEAP and the calculated infrared spectrum (Figure 9). The results from Figure 9 were summarized in Table 3. Upon comparison of Tables 3 and 1, it was evident that both spectra were similar, albeit with minor differences in signal intensity. These discrepancies could be attributed to variations in the states of the materials used in the experimental and computational procedures [32].

Table 2: The calculated structural data of TAEAEAP

Table 3: Comparative analysis of experimental and calculated infrared spectra for TAEAEAP

Figure 8: The optimized conformation of TAEAEAP (by removing hydrogens) from M06-2x of Gaussian

Figure 8: Frequency diagram obtained from DFT calculations

Docking studies

We performed a docking study using AutoDock Vina (v1.2.0) [33,34] and Chimera 15.1 for 3NUP (CDK6 monomeric complex with the inhibitor) and 1BD8. 3NUP is a transferase/transferase inhibitor. Figure 10 shows the best pose with the lowest energy mode of interaction between TAEAEAP and 3NUP. The hydrogen bond length between TAEAEAP and THR267 was approximately 1.887 Å. Figure 11 shows the lowest energy hydrogen bond when studying the molecular docking between the tumor suppressor 1BD8 and TAEAEAP. The hydrogen bond distance between TAEAEAP and MET1 was 2.089 Å.

Figure 10: Molecular docking of TAEAEAP with 3NUP

Figure 11: Molecular docking of TAEAEAP with 1BD8             

Metallic ion sensing characteristics

The fluorescence emission of TAEAEAP was observed at 432 nm when excited at 400 nm. The phosphazene derivative compound was tested for its chemical sensing properties by adding four metal ions (Pb ^{2 +}, Cd^{2 +}, Cu^{2 +}, and Ni ²⁺) to a mixture of (9:1 v/v) H₂O/methanol (Figure 12). The fluorescence emission of TAEAEAP increased with the addition of Cd²⁺ and Pb²⁺ and decreased with the addition of Ni²⁺ and Cu²⁺.

Cytotoxicity of [N₃P₃(OC₂H₅NC₂H₅N)₂]

The biocompatibility of TAEAEAP was thoroughly assessed through cell viability assays employing L929 fibroblast cells. The MTT assay, a widely recognized method for evaluating cytotoxicity, was conducted across a spectrum of TAEAEAP concentrations ranging from 7.8125 to 1000 g/mL. The results, as illustrated in Figure 13, unequivocally demonstrate that TAEAEAP exhibited no significant cytotoxic effects on the L929 fibroblast cells within the tested concentration range. In addition, the WST-1 assay further corroborated these findings, affirming the compound's compatibility with healthy cell lines.

In addition to manifesting biological compatibility, as evidenced by the findings elucidated in the cytotoxicity assessment and the subsequent elucidation of metallic ion sensing characteristics in Section 3.7, TAEAEAP exhibits auspicious attributes in terms of environmental compatibility and chemical sensing properties.

Figure 12: Fluorescence changes of the solution containing TAEAEAP and 10 equiv of addition of Pb ²⁺, Cd²⁺, Cu²⁺, and Ni²⁺ in room temperature

Figure 13: Cytotoxicity effect of TAEAEAP on fibroblast cells

Antibacterial assay

We tested the antibacterial activity of compound (TAEAEAP)₂Cu(NO₃)₂ against Staphylococcus aureus ATCC 6538 (G+), and Bacillus subtilis 2a ATCC 23857 G(+) bacteria using the disk diffusion method [35].  Four concentrations (2, 5, 10, and 20 mg/mL) of compound in DMSO were applied to filter paper disks. The disks were placed on agar plates with bacterial strains and incubated at 37 °C for 24 hours. The zones of inhibition around the disks were measured to determine the effectiveness of the compound (Table 4).

Table 4: The measurements of inhibition diameters around the disks of antibacterial assay

Conclusion

In conclusion, the evaluation of the cytotoxicity of TAEAEAP using L929 fibroblast cells demonstrated no toxicity at the given concentrations. The cytotoxic response of cell lines and the inhibitory effect of the compound on growth were assessed using the MTT assay. The presence of the phosphazene ring in the product and precursors was confirmed by the IR spectra, which exhibited peaks corresponding to the asymmetric and symmetric stretching vibrations of P=N and P-N, respectively. The absence of the OH peak and the presence of the amino group peak further indicated the replacement of chlorine by amino alcohols on phosphorus. The UV spectrum of TAEAEAP exhibited two absorbance bands at 295 nm and 345 nm, attributed to π-π* transitions of the phosphazene ring and intramolecular charge transfer within the 2-[(2-aminoethyl) amino] ethanol moiety, respectively.

Furthermore, the fluorescence properties of the oily cyclotriphosphazene derivatives were examined, revealing a fluorescence band at approximately 488 nm. Density functional theory (DFT) calculations provided insight into the structure of TAEAEAP, which consisted of heterocyclic molecules attached to the N₃P₃Cl₆ core via distorted 8-membered rings. Docking studies using AutoDock Vina and Chimera 15.1 elucidated the interactions between TAEAEAP and the transferase/transferase inhibitor 3NUP, as well as the tumor suppressor 1BD8, highlighting the formation of hydrogen bonds with specific amino acid residues.

We have investigated the synthesis, structure, and antibacterial activity of the (TAEAEAP)₂Cu(NO₃)₂ complex, which exhibits a striking purple color. The complex has proven to be a potent antimicrobial agent against the Staphylococcus aureus ATCC 6538 and Bacillus subtilis 2a ATCC 23857 strains. Our study has established a direct relationship between the concentration of the complex and its ability to inhibit the bacterial growth.

Acknowledgements

We are grateful to Payam Noor University of Iran for laboratory support.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for     profit sectors.      

Conflict of interest

There are no conflicts of interest to disclose.

ORCID

Camelia Gholamrezazadeh

https://orcid.org/0000-0002-1960-8679

Mohammad Hakimi

https://orcid.org/0000-0001-8179-1622

Mehdi Dadmehr

https://orcid.org/0000-0002-6016-5988

HOW TO CITE THIS ARTICLE

Camelia Gholamrezazadeh, Mohammad Hakimi*, Mehdi Dadmehr. A New and Safe Spirocyclic Alkoxy Phosphazene: Synthesis, Characterization, DFT, Molecular Docking and Photophysical Properties. Chem. Methodol., 2023, 7(12) 944-963.

DOI: https://doi.org/10.48309/chemm.2023.422145.1735

URL: https://www.chemmethod.com/article_183865.html