A Study of the Antioxidant and Antibacterial Effect of Dichloromethane Roots Extract of Salvia larifolia and Salvia atropatana Medicinal Plants

Hassani, Hassan; Asili, Javad; Rafatpanah Baygi, Roxana; Dadmehr, Mehdi

doi:10.48309/chemm.2026.558455.2041

A Study of the Antioxidant and Antibacterial Effect of Dichloromethane Roots Extract of Salvia larifolia and Salvia atropatana Medicinal Plants

Document Type : Original Article

Authors

Hassan Hassani ¹

Javad Asili ²

Roxana Rafatpanah Baygi ¹

Mehdi Dadmehr ¹

¹ Department of Chemistry, Payam Noor University, Tehran, Iran

² Mashhad University of Medical Sciences, Department of Pharmacology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

https://doi.org/10.48309/chemm.2026.558455.2041

Abstract

Regarding to the importance of bacterial resistance to many antibiotics and due to the adverse nutritional and carcinogenic effects of many synthetic antioxidants, the discovery and development of antibacterial and antioxidant ag ents derived from plants have been of interest in recent years. This study was conducted on two species of Salvia to determine their antibacterial and antioxidant properties. The antibacterial effect of dichloromethane extract of the roots of Salvia larifolia and Salvia atropatana was studied using disk diffusion method. Additionally, three methods DPPH, FRAP, and BCB were used to evaluate the antioxidant power of the extracts. The results of this study showed that the extracts at concentrations of 40 and 80 µg/mL had the highest antibacterial and antioxidant properties, respectively. The effect of the extracts on gram-positive bacteria was greater than on gram-negative bacteria. The antioxidant power of Salvia larifolia was higher than that of Salvia atropatana in the DPPH and FRAP methods, but lower at some concentrations in the BCB method, indicating a difference in the amount of compounds present in these two species. The results of this study suggest that the studied species can be used as effective natural antibacterial and antioxidant agents in various industries.

Graphical Abstract

Keywords

Salvia larifolia

Salvia atropatana

Antibacterial

Antioxidant

Subjects

Biochemistry

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[1] Khalil, A., Hassawi, D.S., Kharma, A. Genetic relationship among Salvia species and antimicrobial activity of their crude extract against pathogenic bacteria. Asian Journal of Plant Sciences, 2025, 4(5), 544-549.

[2] Nascimento, G.G., Locatelli, J., Freitas, P.C., Silva, G.L. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology, 2000, 31, 247-256.

[3] Hsueh, P.R. World Health Day 2011–antimicrobial resistance: No action today, no cure tomorrow. Journal of the Formosan Medical Association, 2011, 110(4), 213-214.

[4] Savoia, D. Plant-derived antimicrobial compounds: alternatives to antibiotics. Future Microbiology, 2012, 7(8), pp.979-990.

[5] Abdel-Hady, H., El-Wakil, E.A., Abdel-Gawad, M. GC-MS analysis, antioxidant and cytotoxic activities of Mentha spicata. European Journal of Medicinal Plants, 2018, 26(1), 1-12.

[6] Abdul, W.M., Hajrah, N.H., Sabir, J.S., Al-Garni, S.M., Sabir, M.J., Kabli, S.A., Saini, K.S., Bora, R.S. Therapeutic role of Ricinus communis L. and its bioactive compounds in disease prevention and treatment. Asian Pacific Journal of Tropical Medicine, 2018, 11(3), 177-185.

[7] Halliwell, B., Aeschbach, R., Löliger, J., Aruoma, O.I. The characterization of antioxidants. Food and Chemical Toxicology, 1995, 33(7), 601-617.

[8] Mossi, A.J., Cansian, R.L., Paroul, N., Toniazzo, G., Oliveira, J.V., Pierozan, M.K., Pauletti, G., Rota, L., Santos, A.C.A., Serafini, L.A. Morphological characterisation and agronomical parameters of different species of Salvia sp. (Lamiaceae). Brazilian Journal of Biology, 2011, 71, 121-129.

[9] Albaladejo, R.G., Aparicio, A., Silvestre, S. Variation patterns in the Phlomis× composita (Lamiaceae) hybrid complex in the Iberian Peninsula. Botanical Journal of the Linnean Society, 2004, 145(1), 97-108.

[10] Kharazian, N. Identification of flavonoids in leaves of seven wild growing Salvia L. (Lamiaceae) species from Iran. Progress in Biological Sciences, 2013, 3(2), 81-98.

[11] Hamlyn. P. The marshall cavendish. Encyclopedia of Gardening,1969.

[12] Anačkov, G., Božin, B., Zorić, L., Vukov, D., Mimica-Dukić, N., Merkulov, L., Igić, R., Jovanović, M., Boža, P. Chemical composition of essential oil and leaf anatomy of Salvia bertolonii Vis. and Salvia pratensis L. (Sect. Plethiosphace, Lamiaceae). Molecules, 2008, 14(1),1-9.

[13] Hosseinpoor, M.A.Z., Mahdavi, B., Rezaei, S.E. 2016. Contents of aerial parts of Salvia leriifolia benth. Journal of Chemical Health Risks Year, 2016, 6(3), 185-194.

[14] Hosseinzadeh, H., Lary, P. Effect of Salvia leriifolia leaf extract on morphine dependence in mice. Phytotherapy Research, 2000, 14(5), 384-387.

[15] Baydoun, E., Bibi, M., Iqbal, M.A., Wahab, A.T., Farran, D., Smith, C., Sattar, S.A., Rahman, A.U., Choudhary, M.I. Microbial transformation of anti-cancer steroid exemestane and cytotoxicity of its metabolites against cancer cell lines. Chemistry Central Journal, 2013, 7(1), 57.

[16] Sadeghnia, H., Nassiri Asl, M., Haddadkhodaparast, M., Hosseinzadeh, H. The effect of Salvia leriifolia Benth. root extracts on lipid peroxidation level during global ischemic-reperfusion in rats, Journal of Medicinal Plants. 2003, 2(7), 19-28.

[17] Salimikia, I., Monsef-Esfahani, H.R., Gohari, A.R., Salek, M. Phytochemical analysis and antioxidant activity of Salvia chloroleuca aerial extracts. Iranian Red Crescent Medical Journal, 2016, 18(8), 24836.

[18] Firuzi, O., Miri, R., Asadollahi, M., Eslami, S., Jassbi, A.R. Cytotoxic, antioxidant and antimicrobial activities and phenolic contents of eleven Salvia species from Iran. Iranian Journal of Pharmaceutical Research: IJPR, 2013, 12(4), 801.

[19] Derakhshan, Z., Ferrante, M., Tadi, M., Ansari, F., Heydari, A., Hosseini, M.S., Conti, G.O., Sadrabad, E.K. Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds. Food and Chemical Toxicology, 2018, 114, 108-111.

[20] Daneshzadeh, M.S., Abbaspour, H., Amjad, L., Nafchi, A.M. An investigation on phytochemical, antioxidant and antibacterial properties of extract from Eryngium billardieri F. Delaroche. Journal of Food Measurement and Characterization, 2020, 14(2), 708-715.

[21] Corrales, M., García, A.F., Butz, P., Tauscher, B. Extraction of anthocyanins from grape skins assisted by high hydrostatic pressure. Journal of food Engineering, 2009, 90(4), 415-421.

[22] Reda, M., Migocka, M., Kłobus, G. Effect of short-term salinity on the nitrate reductase activity in cucumber roots. Plant Science, 2011, 180(6), 783-788.

[23] Walker, J.B., Sytsma, K.J. Staminal evolution in the genus Salvia (Lamiaceae): molecular phylogenetic evidence for multiple origins of the staminal lever. ̶Annals of Botany, 2007, 100(2), 375-391.

[24] Mangena, T., Muyima, N.Y.O. Comparative evaluation of the antimicrobial activities of essential oils of Artemisia afra, Pteronia incana and Rosmarinus officinalis on selected bacteria and yeast strains. Letters in applied microbiology, 1999, 28(4), 291-296.

[25] Yamashoji, S., Kajimoto, G. Antioxidant effect of Gly-Gly-His on Cu (II)-catalyzed autoxidation and photosensitized oxidation of lipids. Agricultural and Biological Chemistry, 1980, 44(11), 2735-2736.

[26] Brand-Williams, W., Cuvelier, M.E., Berset, C.L.W.T. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 1995, 28(1), pp.25-30.

[27] Mancuso, M., Pacini, M., Gemignani, P., Bartalini, B., Agostini, B., Ferroni, L., Cantagallo, A. Clinical application of prismatic lenses in the rehabilitation of neglect patients. A randomized controlled trial. European Journal of Physical and Rehabilitation Medicine, 2012, 48(2), 197-208.

[28] Benzie, I.F., Strain, J.J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 1996, 239(1), 70-76.

[29] Unten, L., Koketsu, M., Kim, M. Antidiscoloring activity of green tea polyphenols on β-carotene. Journal of Agricultural and food Chemistry, 1997, 45(6), 2009-2012.

[30] Moreno, F.J., Corzo-Martı, M., Del Castillo, M.D., Villamiel, M. Changes in antioxidant activity of dehydrated onion and garlic during storage. Food Research International, 2006, 39(8), 891-897.

[31] Aruoma, O.I. Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2003, 523, 9-20.

[32] Wu, Y., Deng, Y., Li, Y. Changes in enzyme activities in abscission zone and berry drop of ‘Kyoho’grapes under high O₂ or CO₂atmospheric storage. LWT-Food Science and Technology, 2008, 41(1), 175-179.

[33] Nouri, L., Nafchi, A.M. Antibacterial, mechanical, and barrier properties of sago starch film incorporated with betel leaves extract. International Journal of Biological Macromolecules, 2014, 66(254-259.

[34] Pesek, C., Warthesen, J. Characterization of the photodegradation of β‐carotene in aqueous model systems. Journal of Food Science, 1988, 53(5), 1517-1520.

[35] Sun, J., Chu, Y.F., Wu, X., Liu, R.H. Antioxidant and antiproliferative activities of common fruits. Journal of Agricultural and food Chemistry, 2002, 50(25), 7449-7454.

[36] Menichini, F., Tundis, R., Bonesi, M., Loizzo, M.R., Conforti, F., Statti, G., Menichini, F. The influence of fruit ripening on the phytochemical content and biological activity of Capsicum chinense Jacq. cv Habanero. Food Chemistry, 2009, 114(2), 553-560.